global stem cells group | Stem Cells Course

Tag Archives for " global stem cells group "

How much does exosome therapy cost?

Exosome therapy is the new buzz in the regenerative medicine industry because of how it can repair and regenerate your cells and tissues.

Exosome therapy is safer compared to other cellular therapy because it’s a cell-free therapy with no risk of rejection.

Exosome therapy will be beneficial to you if you’re dealing with conditions such as sport injuries, tissue regeneration,  hair loss, erectile dysfunction, chronic pain and so many other applications . 

In this article, you’ll be learning the cost of exosome therapy and how you can benefit from exosome therapy.

How the cost of exosome therapy is determined

All cells produce exosomes, which are microvesicles that contain biochemical and genetic information. 

Hence, the cost of an exosome product (used in exosome therapy) will depend on what type of cell line (raw tissue source) used to extract the exosomes.

The first factor to determine the cost of an exosome product depends on the quality of the tissue source.

The most commonly used innovative design tissue types are cord blood, amniotic fluid and mesenchymal cell cultures.

Exosomes derived from mesenchymal cell cultures are the most difficult to obtain but offer the greatest therapeutic potential.

How much does exosome therapy cost?

The average cost of exosome therapy is $4,900, but the price can be anything from $3,500 – $6,500.

It’s also important to note that the price depends on your specific needs and your treatment plan, as decided by the doctor.

The doctor will schedule a consultation with you to determine your personalized treatment plan.

The exosome therapy can either be given as an IV infusion or as localized injections, depending on the purpose of the therapy.

Exosomes are very useful to revitalize, rejuvenate, restore, and reduce inflammations in the body.

Here are some ways you can benefit from exosome therapy

Hair loss therapy: If you’re in the early stages of hair loss, with exosome therapy you can regenerate your hair whether you’re a man or woman. After exosome therapy, you’ll start seeing new hair growth in as little as two to three months with very significant results showing 6 months or 1 year later.

Chronic pain: In case you’re experiencing chronic pain due to degenerative conditions such as arthritis, exosomes can help to subdue the pain by regenerating the cells and helping the body work better.

Degenerative conditions: If you’re struggling with degenerative medical conditions such as osteoarthritis and musculoskeletal injuries, exosome therapy can help your body repair the damage done to your cells by these conditions, prevent them from getting better, and help you to feel better.

Skin therapy: exosome therapy can reduce inflammation in the skin by improving the strength and elasticity of the skin.

Anti-aging: if you would like to retain your youthful glow, exosome therapy can make you feel young again by rejuvenating your skin due to its ability to reverse the cells dying due to aging.

Where can you get exosome therapy?

Cellular hope institutes provide exosome therapy for patients looking for better outcomes for various conditions.

The exosomes used at Cellular Hope Institute with are obtained from umbilical cord tissue that is discarded after a new birth , which means these Exosomes have not been exposed to any contaminating or toxic agent because our cells are as healthy as our body. This gives it a higher capacity to regenerate your cells and tissues.

Culture Expanded MSCs

Mesenchymal Stem Cells (MSCs) are the most commonly used cells in stem cell therapy and regenerative medicine, due to their high and multi-potency. Mesenchymal Stem Cells (MSCs) can be isolated from different tissues in the body.

In this article, you’ll be learning about culture-expanded MSCs, how MSCs can be expanded, The potency of MSCs and the type of cells they can differentiate into.

What are culture expanded Mesenchymal Stem cells?

Mesenchymal stem cells are high potent cells used for cellular therapy and isolated from different parts of the body. Mesenchymal stem cells can be used to improve the patient outcome in diseases and conditions such as autoimmune diseases, degenerative diseases, nerve damage, diabetes mellitus, bone problems etc.

For every patient, millions of mesenchymal stem cells are needed and the exact amount varies according to disease, route of administration, administration frequency, weight, and age of patient.

Mesenchymal stem cells are expanded in a culture media, on a large scale in order to obtain the required quantity of cells needed for cellular therapy.

Culture expanded MSCs: How does it work?

Expanding Mesenchymal stem cells in a media involves step by step process of isolation and expansion.

Mesenchymal Stem Cells Isolation

Mesenchymal stem cells can be isolated from different tissues in the human body such as adipose tissues, dental pulp, human bone marrow, umbilical cord tissue, umbilical cord blood, peripheral blood and synovium.

Mesenchymal stem cells are expanded in culture to increase their yield and amplify their desired functions and potency.

Although the population of Mesenchymal Stem Cells obtained will vary from donor to donor, here are some steps to follow:

· Acquire fresh tissue extracts in strictly aseptic conditions, to maintain purity.

· To remove any cell clusters, you have to filter the cell suspension with a 70-mm filter mesh

· Use a centrifuge to roll the cells for about 5 minutes at 500g

·  Suspend the cells again the cells to measure the cell viability and yield using Trypan blue exclusion

· Use in T75 culture dishes to culture the cells in 10 mL of complete MSC medium at a density of 25 × 106 cells/mL. You can then go on to Incubate the plates at 37 °C with 5% CO2 in a humidified chamber without any interruption.

· When it’s past 3 h, remove the non-adherent cells that accumulate on the surface of the dish by changing the medium and replacing it with 10 mL fresh complete medium.

·  After an additional 8 h of culture, add 10ml fresh complete medium as a replacement for the existing medium. You’ll have to repeat this step every 8 h for up to 72 h of initial culture.

· Cells can be frozen in MSC growth media plus 10% DMSO (D2650) at a density of 2X106 cells/vial.

Expansion of Mesenchymal Stem Cells in a culture media

Culture expanded mesenchymal cells undergo various stages from the preparation of the culture plate, thawing of Mesenchymal stem cells, and the actual expansion of Mesenchymal stem cells.

The reason behind the cultural expansion of Mesenchymal stem cells is to get them to differentiate into other cell types such as osteoblast, adipocyte, and mesenchymal stromal cells.

In preparation, to expand MSCs in a culture media, you need a culture ware. You can get one plastic or glassware plate and coat it with a sufficient amount of 0.1% gelatin. Don’t forget to aspirate the gelatin solution from the coated plate or flask before you use it.

The next step involves the thawing of the Mesenchymal stem cells, and here are a few steps for you to follow:

After the recommended culture medium and coated culture ware is ready and on standby, remove the vial of Mesenchymal Stem Cells from liquid nitrogen and incubate in a 37C water bath and pay close attention to it, until all the cells are completely thawed. The extent of completely thawed frozen cells and how fast, are what determines the cell viability.

Once the cells have thawed completely, take steps to avoid contamination by disinfecting the walls with 70% ethanol, before you proceed to the next step.

Place the cells in a hood, and carefully transfer the cells to a sterile tube with a pipette (1 or 2ml pipette), Do this in such a way to prevent bubbles.

Then, add drops of Mesenchymal Stem cell expansion medium that have been pre-warmed to 37C to the tube containing the Mesenchymal stem cells.

Be careful to take your time when adding the medium to avoid osmotic shock which could lead to decreased viability.

Proceed to mix the suspension slowly by pipetting up and down two times while avoiding any bubbles.

Place the tube in a centrifuge and centrifuge the tube at 300 x g for 2-3 minutes to roll the cells, and you should not vortex the cells.

After this, then decant as much of the supernatant as possible. These steps are necessary to remove residual cryopreservative (DMSO).

Suspend the cells in a total volume of 10 mL of Mesenchymal Stem Cell Expansion Medium again or any alternative of choice, pre-warmed to 37 °C, containing freshly added 8 ng/mL FGF-2 (F0291).

The next step involves placing the cell suspension onto a 10-cm tissue culture plate or a T75 tissue culture flask.

Maintain the cells in a humidified incubator at 37 °C  with 5% CO2.

The next day, exchange the medium with fresh Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37 °C) containing 8 ng/mL FGF-2*. Replace with fresh medium containing FGF-2 every two to three days thereafter.

Isolate the cells when they are approximately 80% confluent, using Trypsin-EDTA and passaged further or frozen for later use.

Expansion of Mesenchymal Stem Cells

Once the cells are actively proliferating and have reached a confluence of approximately 80% (before 100%), you should subculture the cells.

Then remove the medium from the 10-cm tissue culture plate containing the confluent layer of human mesenchymal stem cells, carefully and apply 3-5 mL of Trypsin-EDTA Solution, before proceeding to incubate in a 37 °C incubator for 3-5 minutes.

Crosscheck the culture to see if all the cells are completely detached. Then, add 5 mL Mesenchymal Stem Cell Expansion Medium to the plate.

Swirl the plate mildly to mix the cell suspension. Transfer the separated/isolated cells to a 15 mL conical tube.

Centrifuge the tube at 300 x g for 3-5 minutes to pellet the cells.

Throw the supernatant away and apply 2 mL Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37 °C) containing 8 ng/mL FGF-2 to the conical tube and completely suspend the cells again. Remember not to vortex the cells.

Then, use a hemocytometer to count the number of cells.

Plate the cells at a density of 5,000-6,000 cells per cm2 into the appropriate flasks, plates, or wells in a Mesenchymal Stem Cell Expansion Medium containing 8 ng/mL FGF-2.

Cells can be frozen in MSC growth media plus 10% DMSO (D2650) at a density of 2X106 cells/vial.

Functions of Culture Expanded MSCs

Mesenchymal stem cells are required to be expanded in order for them to be used clinically for therapeutic purposes.

The culture expanded MSCs can be induced to differentiate into adipocytes, osteocytes, hepatocytes, chondrocytes, tenocytes and cardiomyocytes.

Because of its potential to differentiate into different kinds of cells in the body, it can be used to manage liver problems, heart problems, joint and bone problems etc.

Mesenchymal stem cells are also used in tissue regeneration and modulation of the immune system. They possess anti apoptotic, angiogenic, anti fibrotic, and anti-oxidative properties.

However, the quantity of MSCs isolated from body tissues is not enough for clinical and therapeutic applications.

This is why MSCs are expanded in culture to increase their yield for desired therapeutic effect.

Why cellular therapies have become a standard in clinics that are betting on biological medicine

Cellular therapy is fast becoming a standard therapy in many regenerative clinics today.  Many doctors are no longer questioning the safety and effectiveness of stem cell therapy. This is because various stem cell studies are already describing the benefits of stem cells for patients who are living with chronic and autoimmune health conditions.

This article will be talking about why stem cell therapy have become a standard therapy in clinics, the paracrine effect of stem cells, and other reasons why doctors are adopting stem cells in their clinics.

Benefits of stem cell therapy

Stem cell therapy is an important innovation in medicine because of its regenerative power in the human body. Most disease states are characterized by damaged cells, tissues and organs, which is where stem cell therapy comes in. In stem cell therapy, stem cells are administered into the human body and it replaces the cells damaged by disease or health disorders.

Stem cell research has revealed two major ways of using stem cells to rebuild defective and damaged cells. One of these ways can be seen in procedures like bone marrow transplant, where stem cells are used to replace the damaged cells by engraving, and they then differentiate into the proper cell type. Another mechanism relies on the paracrine effect of stem cells. This procedure of stem cell therapy involves using stem cells isolated from a donor to stimulate the patient’s cells to repair damaged tissues.

Additionally, unlike traditional therapy, stem cells have a wide application. Stem cell therapy is used to manage various degenerative diseases, autoimmune disorders, birth defects, and the research is still ongoing for so many other health conditions where stem cells have shown potential.

Also, there is currently a high demand for aesthetic medicine. Stem cell therapy is a proven alternative to other forms of cosmetology such as plastic surgery. Hence, dermatologists are turning to stem cell therapy to administer anti-aging procedures, skin rejuvenation, hair therapy, micro-needling etc.

The Paracrine effect of stem cells

The paracrine effect of stem cells is one of the most outstanding effects of stem cells. It involves using donor cells to stimulate endogenous repair by harnessing the regenerative power of the human body. It is a mechanism of tissue regeneration that has created new possibilities for managing various conditions using stem cell therapy.

The cells that trigger a paracrine response are; mesenchymal cells, umbilical cord blood, umbilical cord tissue, adipose (fat) tissue and blood cells from a donor’s bone marrow.

The paracrine effect occurs when the donor’s cells send the damaged or defective cells signals to induce self regeneration and repair by secreting some factors and proteins. One of the mechanism by which this paracrine effect is initiated, involves the secretion of cytokines and regulatory proteins by the damaged patient’s cells, these cytokines and proteins act as mediators to stimulate an immune response that attracts the donor cells, this causes the donor cells to release proteins and factors that stimulate the patient’s cells to promote cell proliferation, increase vascularization and blood flow to the areas that needs to heal, while reducing inflammation.

Moreover, research has shown that the paracrine effect of stem cells prevents damaged and diseased cells from dying. They are also therapeutically useful in autoimmune diseases and preventing transplant rejection due to the immune suppression effect they have.

Is stem cell therapy effective?

Doctors are always looking for ways to provide the best possible treatment to their patients, and that is why many clinics are embracing stem cell therapy as a standard, due to its many advantages.

Stem cell therapy is one of the most effective and safest therapy patients can receive, when compared to other existing treatment options.  Stem cell therapy is used in promoting patient outcomes in a lot of disease conditions that were previously poorly treated by other alternatives.

Again, as new potentials and ways of applying stem cells are being discovered, doctors are beginning to maximize these benefits in their clinics. Some conditions that are currently treated by stem cells include autoimmune conditions, immunotherapy Car-T cells, chronic obstructive pulmonary disease, neurodegenerative conditions, osteoarthritis, spinal cord injury, aesthetics/anti-aging, sports medicine, autism and multiple sclerosis.

Another reason clinics are adopting stem cell therapy as a standard therapy is because it is easy to administer. A lot of machines such as GCELL {Insert link} which makes the harvesting and processing of stem cells easy and fast, have made the procedures easily adaptable by doctors.

Furthermore, stem cell therapy reduces the treatment and recovery time associated with surgical procedures and other treatment options. This alone is a big factor in why stem cells are becoming a standard therapy in clinics.

Therapeutic uses of stem cells vs traditional medicine

Existing stem cell research has shown how the regenerative effect of stem cells is defining the future of medicine. The major advantage of stem cell therapy over conventional medication-based therapy is its safety. Stem cell therapy is aimed at treating the cause of the disease while traditional medicine targets the symptoms.

 Another problem with traditional medical therapy is that it introduces another problem while trying to solve the existing one. As a doctor, you always run the risk of causing harm with each prescription because of various adverse effects that could lead to major organ damage of the kidney, liver etc.  On the other hand, patients already know this and they are actively seeking better alternatives, this is why stem cell therapy is fast becoming a standard therapy in clinics.

Moreover, doctors will always be concerned about whether their patients are taking their medications or not. The burden of drug compliance and adherence associated with traditional medical therapy is not always easy to navigate. This is why effective treatment options like stem cell therapy have become a standard therapy in clinics. It only requires the patients having a procedure that repairs and restores damaged cells and tissues in the most natural way.

If you would like to become certified in regenerative medicine using stem cells and other cellular therapy, contact us.

1 Enrique Testart, M.D.

Enrique Testart, M.D.

Enrique Testart, M.D., Global Stem Cells Group’s Chief Medical Officer (CMO), is a surgeon specializing in child trauma microsurgery.

Dr. Testart is also a medical entrepreneur and founder of Consortia Innovas S.A. in Santiago, Chile, dedicated to consulting and clinical health management for clinical management firms and research and development-oriented planners in the latest treatments in regenerative medicine as they become available.

A native of Santiago, Chile, Dr. Testart’s medical studies took him all over the world, including orthopedics studies under the direction of Prof. Jean Paul Metaizeau, M.D. in France.

He is in charge of all Global Stem Cells Group divisions and programs in Chile, including patient recruitment through Cellgenic, medical training and certification through Stem Cell Training Inc., and everything related to the sale of equipment disposable through Adimarket.

Julio Ferreira advisory board, Global Stem Cells Group

Julio Ferreira, M.D.

Julio Ferreira, M.D.
Global Stem Cells Group Advisory Board member

Julio Ferreira, M.D., is an internationally recognized and respected cosmetic surgeon and professor of medicine and aesthetic surgery at the Institute of Biomedical Sciences, University of Sao Paulo, Brazil;

As director of Clinica Ferreira in Argentina, Dr. Ferreira is dedicated to the combination of art and science in aesthetic medicine.

Dr. Ferreira serves as president of the South American Academy of Cosmetic Surgery and expert examiner at the International Board of Cosmetic Surgery. He also serves on the International Editorial Advisory Board of the American Journal of Cosmetic Surgery; former President of the International Academy of Cosmetic Surgery 2005/2007; a member and examiner, International Board of Cosmetic Surgery; Corresponding Fellow of the American Academy of Cosmetic Surgery; Honorary Member of the Spanish Society of Cosmetic Medicine and Surgery; Honorary Member of the Eurorusa Confederation of Societies of Aesthetic Plastic Surgery; Honorary Member of the Bulgarian Society of Cosmetic Surgery; Honorary Member of the Chilean Society of Cosmetic Surgery and Lipoplasty; Honorary Member of the Italian Society of Aesthetic Surgery, and Honorary Member of the French Society of Aesthetic Surgery.

 

adana escort , adıyaman escort , afyon escort , ağrı escort , aksaray escort , amasya escort , ankara escort , antalya escort , ardahan escort , artvin escort , aydın escort , balıkesir escort , bartın escort , batman escort , bayburt escort , bilecik escort , bingöl escort , bitlis escort , bolu escort , burdur escort , bursa escort , çanakkale escort , çankırı escort , çorum escort , denizli escort , diyarbakır escort , düzce escort , edirne escort , elazığ escort , erzincan escort , erzurum escort , eskişehir escort , gaziantep escort , giresun escort , gümüşhane escort , hakkari escort , hatay escort , ığdır escort , ısparta escort , istanbul escort , izmir escort , karabük escort , karaman escort , kars escort , kastamonu escort , kayseri escort , kilis escort , kırıkkale escort , kırklareli escort , kırşehir escort , kocaeli escort , konya escort , kütahya escort , malatya escort , manisa escort , maraş escort , mardin escort , mersin escort , muğla escort , muş escort , nevşehir escort , niğde escort , ordu escort , osmaniye escort , rize escort , sakarya escort , samsun escort , şanlıurfa escort , siirt escort , sinop escort , şırnak escort , sivas escort , tekirdağ escort , tokat escort , trabzon escort , tunceli escort , uşak escort , van escort , yalova escort , yozgat escort , zonguldak escort , kadıköy escort , şişli escort , tuzla escort , bornova escort , didim escort , serdivan escort , escort