regenerative medicine training | Stem Cells Course

Tag Archives for " regenerative medicine training "

How much does exosome therapy cost?

Exosome therapy is the new buzz in the regenerative medicine industry because of how it can repair and regenerate your cells and tissues.

Exosome therapy is safer compared to other cellular therapy because it’s a cell-free therapy with no risk of rejection.

Exosome therapy will be beneficial to you if you’re dealing with conditions such as sport injuries, tissue regeneration,  hair loss, erectile dysfunction, chronic pain and so many other applications . 

In this article, you’ll be learning the cost of exosome therapy and how you can benefit from exosome therapy.

How the cost of exosome therapy is determined

All cells produce exosomes, which are microvesicles that contain biochemical and genetic information. 

Hence, the cost of an exosome product (used in exosome therapy) will depend on what type of cell line (raw tissue source) used to extract the exosomes.

The first factor to determine the cost of an exosome product depends on the quality of the tissue source.

The most commonly used tissue types are cord blood, amniotic fluid and mesenchymal cell cultures.

Exosomes derived from mesenchymal cell cultures are the most difficult to obtain but offer the greatest therapeutic potential.

How much does exosome therapy cost?

The average cost of exosome therapy is $4,900, but the price can be anything from $3,500 – $6,500.

It’s also important to note that the price depends on your specific needs and your treatment plan, as decided by the doctor.

The doctor will schedule a consultation with you to determine your personalized treatment plan.

The exosome therapy can either be given as an IV infusion or as localized injections, depending on the purpose of the therapy.

Exosomes are very useful to revitalize, rejuvenate, restore, and reduce inflammations in the body.

Here are some ways you can benefit from exosome therapy

Hair loss therapy: If you’re in the early stages of hair loss, with exosome therapy you can regenerate your hair whether you’re a man or woman. After exosome therapy, you’ll start seeing new hair growth in as little as two to three months with very significant results showing 6 months or 1 year later.

Chronic pain: In case you’re experiencing chronic pain due to degenerative conditions such as arthritis, exosomes can help to subdue the pain by regenerating the cells and helping the body work better.

Degenerative conditions: If you’re struggling with degenerative medical conditions such as osteoarthritis and musculoskeletal injuries, exosome therapy can help your body repair the damage done to your cells by these conditions, prevent them from getting better, and help you to feel better.

Skin therapy: exosome therapy can reduce inflammation in the skin by improving the strength and elasticity of the skin.

Anti-aging: if you would like to retain your youthful glow, exosome therapy can make you feel young again by rejuvenating your skin due to its ability to reverse the cells dying due to aging.

Where can you get exosome therapy?

Cellular hope institutes provide exosome therapy for patients looking for better outcomes for various conditions.

The exosomes used at Cellular Hope Institute are obtained from umbilical cord tissue that is discarded after a new birth , which means these Exosomes have not been exposed to any contaminating or toxic agent because our cells are as healthy as our body. This gives it a higher capacity to regenerate your cells and tissues.

Culture Expanded MSCs

Mesenchymal Stem Cells (MSCs) are the most commonly used cells in stem cell therapy and regenerative medicine, due to their high and multi-potency. Mesenchymal Stem Cells (MSCs) can be isolated from different tissues in the body.

In this article, you’ll be learning about culture-expanded MSCs, how MSCs can be expanded, The potency of MSCs and the type of cells they can differentiate into.

What are culture expanded Mesenchymal Stem cells?

Mesenchymal stem cells are high potent cells used for cellular therapy and isolated from different parts of the body. Mesenchymal stem cells can be used to improve the patient outcome in diseases and conditions such as autoimmune diseases, degenerative diseases, nerve damage, diabetes mellitus, bone problems etc.

For every patient, millions of mesenchymal stem cells are needed and the exact amount varies according to disease, route of administration, administration frequency, weight, and age of patient.

Mesenchymal stem cells are expanded in a culture media, on a large scale in order to obtain the required quantity of cells needed for cellular therapy.

Culture expanded MSCs: How does it work?

Expanding Mesenchymal stem cells in a media involves step by step process of isolation and expansion.

Mesenchymal Stem Cells Isolation

Mesenchymal stem cells can be isolated from different tissues in the human body such as adipose tissues, dental pulp, human bone marrow, umbilical cord tissue, umbilical cord blood, peripheral blood and synovium.

Mesenchymal stem cells are expanded in culture to increase their yield and amplify their desired functions and potency.

Although the population of Mesenchymal Stem Cells obtained will vary from donor to donor, here are some steps to follow:

· Acquire fresh tissue extracts in strictly aseptic conditions, to maintain purity.

· To remove any cell clusters, you have to filter the cell suspension with a 70-mm filter mesh

· Use a centrifuge to roll the cells for about 5 minutes at 500g

·  Suspend the cells again the cells to measure the cell viability and yield using Trypan blue exclusion

· Use in T75 culture dishes to culture the cells in 10 mL of complete MSC medium at a density of 25 × 106 cells/mL. You can then go on to Incubate the plates at 37 °C with 5% CO2 in a humidified chamber without any interruption.

· When it’s past 3 h, remove the non-adherent cells that accumulate on the surface of the dish by changing the medium and replacing it with 10 mL fresh complete medium.

·  After an additional 8 h of culture, add 10ml fresh complete medium as a replacement for the existing medium. You’ll have to repeat this step every 8 h for up to 72 h of initial culture.

· Cells can be frozen in MSC growth media plus 10% DMSO (D2650) at a density of 2X106 cells/vial.

Expansion of Mesenchymal Stem Cells in a culture media

Culture expanded mesenchymal cells undergo various stages from the preparation of the culture plate, thawing of Mesenchymal stem cells, and the actual expansion of Mesenchymal stem cells.

The reason behind the cultural expansion of Mesenchymal stem cells is to get them to differentiate into other cell types such as osteoblast, adipocyte, and mesenchymal stromal cells.

In preparation, to expand MSCs in a culture media, you need a culture ware. You can get one plastic or glassware plate and coat it with a sufficient amount of 0.1% gelatin. Don’t forget to aspirate the gelatin solution from the coated plate or flask before you use it.

The next step involves the thawing of the Mesenchymal stem cells, and here are a few steps for you to follow:

After the recommended culture medium and coated culture ware is ready and on standby, remove the vial of Mesenchymal Stem Cells from liquid nitrogen and incubate in a 37C water bath and pay close attention to it, until all the cells are completely thawed. The extent of completely thawed frozen cells and how fast, are what determines the cell viability.

Once the cells have thawed completely, take steps to avoid contamination by disinfecting the walls with 70% ethanol, before you proceed to the next step.

Place the cells in a hood, and carefully transfer the cells to a sterile tube with a pipette (1 or 2ml pipette), Do this in such a way to prevent bubbles.

Then, add drops of Mesenchymal Stem cell expansion medium that have been pre-warmed to 37C to the tube containing the Mesenchymal stem cells.

Be careful to take your time when adding the medium to avoid osmotic shock which could lead to decreased viability.

Proceed to mix the suspension slowly by pipetting up and down two times while avoiding any bubbles.

Place the tube in a centrifuge and centrifuge the tube at 300 x g for 2-3 minutes to roll the cells, and you should not vortex the cells.

After this, then decant as much of the supernatant as possible. These steps are necessary to remove residual cryopreservative (DMSO).

Suspend the cells in a total volume of 10 mL of Mesenchymal Stem Cell Expansion Medium again or any alternative of choice, pre-warmed to 37 °C, containing freshly added 8 ng/mL FGF-2 (F0291).

The next step involves placing the cell suspension onto a 10-cm tissue culture plate or a T75 tissue culture flask.

Maintain the cells in a humidified incubator at 37 °C  with 5% CO2.

The next day, exchange the medium with fresh Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37 °C) containing 8 ng/mL FGF-2*. Replace with fresh medium containing FGF-2 every two to three days thereafter.

Isolate the cells when they are approximately 80% confluent, using Trypsin-EDTA and passaged further or frozen for later use.

Expansion of Mesenchymal Stem Cells

Once the cells are actively proliferating and have reached a confluence of approximately 80% (before 100%), you should subculture the cells.

Then remove the medium from the 10-cm tissue culture plate containing the confluent layer of human mesenchymal stem cells, carefully and apply 3-5 mL of Trypsin-EDTA Solution, before proceeding to incubate in a 37 °C incubator for 3-5 minutes.

Crosscheck the culture to see if all the cells are completely detached. Then, add 5 mL Mesenchymal Stem Cell Expansion Medium to the plate.

Swirl the plate mildly to mix the cell suspension. Transfer the separated/isolated cells to a 15 mL conical tube.

Centrifuge the tube at 300 x g for 3-5 minutes to pellet the cells.

Throw the supernatant away and apply 2 mL Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37 °C) containing 8 ng/mL FGF-2 to the conical tube and completely suspend the cells again. Remember not to vortex the cells.

Then, use a hemocytometer to count the number of cells.

Plate the cells at a density of 5,000-6,000 cells per cm2 into the appropriate flasks, plates, or wells in a Mesenchymal Stem Cell Expansion Medium containing 8 ng/mL FGF-2.

Cells can be frozen in MSC growth media plus 10% DMSO (D2650) at a density of 2X106 cells/vial.

Functions of Culture Expanded MSCs

Mesenchymal stem cells are required to be expanded in order for them to be used clinically for therapeutic purposes.

The culture expanded MSCs can be induced to differentiate into adipocytes, osteocytes, hepatocytes, chondrocytes, tenocytes and cardiomyocytes.

Because of its potential to differentiate into different kinds of cells in the body, it can be used to manage liver problems, heart problems, joint and bone problems etc.

Mesenchymal stem cells are also used in tissue regeneration and modulation of the immune system. They possess anti apoptotic, angiogenic, anti fibrotic, and anti-oxidative properties.

However, the quantity of MSCs isolated from body tissues is not enough for clinical and therapeutic applications.

This is why MSCs are expanded in culture to increase their yield for desired therapeutic effect.

GCell: The Newest All-In-One Solution in Regenerative Medicine

Are you a physician that is currently utilizing adipose tissue, but is growing tired of the time-consuming, arduous procedure? Are you having problems finding reagents that allow you to isolate stem cells? If so, GCell might be the solution for you. 

What is GCell? 

GCell is a revolutionary new machine that holds the potential to become the future of adipose stem cell processing. This is due to a variety of reasons, from the compactness of the unit, to its short processing time– it truly is a valuable implement in any regenerative medicine practitioner’s office. GCell employs a process of mechanical breakdown, which in addition to shortening the duration of the treatment, also serves to keep it within legal minimal-manipulation regulations. 

How Does GCell Work? 

Through a system of extremely small blades and filters, it is able to homogenize a sample of fat taken from anywhere in the body, making it into a slurry of growth factors, proteins, and the other components that make up the sample. The GCell then filters out the stromal vascular fraction cells, and uses a process called photoactivation to increase their vitality. Once the process is over, the end result is a final product that can be administered to patients within forty minutes from the sample’s harvesting. This is a far cry from the previous, muti-hour long treatments that physicians have grown accustomed to,  and this shortened timespan is something that both doctors and their patients will greatly appreciate.

regenerative medicine, traumatology

Why GCell? 

Additionally, it is a single-session treatment. This means that patients that wish to receive a GCell treatment will only have to visit their clinic once, and will not have to undergo any followup treatments. This machine provides the convenience of its small size and lightweight build with the ability to accelerate the stem cell harvesting process– but that isn’t all. The GCell has been designed to be an entirely enclosed system– this is important, as it means that samples that are run through the GCell will remain in a sterile environment, which preserves the viability of samples and ensures positive outcomes for patients. 

The GCell combines a grinder, a filter, and a photoactivation device, and is a turnkey solution that can take a physician from harvesting a fat sample to administering an injectable regenerative medicine therapy in less than an hour. GCell has been designed to be especially useful for clinics that are just beginning to implement regenerative medicine into their practice, but it’s all inclusive approach to stem cell isolation is what sets it a cut above the rest.  

regenerative medicine

GCell’s Single Session Treatment Protocol  

Unlike many other cellular therapies on the market, it is a single-session treatment, which means that there is no need for the patient to come back after his original treatment day has ended. This machine’s portability, small stature, and ability to accelerate the stem cell harvesting process makes it ideal for clinics that are just beginning to implement regenerative medicine into their practices, as it takes much of the rigor out of adipose-derived stem cell harvesting.

ISSCA Announces Speaker’s List for 7th Annual Symposium

The International Society for Stem Cells Applications has officially announced and published its list of speakers for its 7th Annual Regenerative Medicine Symposium. The seventh event of its kind, it is another effort from the iSSCA to bring together a network of regenerative medicine practitioners from all over the world. At previous events, hundreds of physicians came to share in their knowledge regarding cellular therapies and the patients whose lives they can change, and the aim of this year is no different– of course, as a reunion of medical professionals, several steps will be taken to ensure proper social distancing measures, as well as frequent opportunities for sanitation. 

As with previous editions of the event, the topics discussed and speakers involved have been updated and edited in an effort to reflect the changing of time and the rapid evolution of the field of regenerative medicine. That is why this symposium will focus on Cellular Therapies During and After the Pandemic. Coronavirus has changed life as we know it, but the scientific and medical communities have not stopped researching. In fact, cellular therapies have been an invaluable asset in managing the largest pandemic in modern history, due to their implications in treating respiratory failure and managing some of the complications induced by exposure to COVID-19.  This has resulted in accelerated pathways for regulatory approval and increased funding for new regenerative medicine-based clinical trials, which will all be discussed in greater depth at the event. 

But the Regenerative Medicine Symposium also provides a valuable networking opportunity for physicians in the Latin American community. “Here, people interested in cellular therapy can find everything they need to get on the right path to helping people with regenerative medicine,” Said Benito Novas, VP of Public Relations, ISSCA, “If you’ve done some research yourself, or really have no idea where to start– this would be the place. We’ve got vendors, instructors, and interview panels with several distinguished researchers,” 

About ISSCA

The International Society for Stem Cells Applications (ISSCA) is a multidisciplinary community of scientists and physicians, all of whom aspire to treat diseases and lessen human suffering through advances in science, technology, and the practice of regenerative medicine. Incorporated under the Republic of Korea as a non-profit entity, the ISSCA is focused on promoting excellence and standards in the field of regenerative medicine.

ISSCA bridges the gaps between scientists and practitioners in Regenerative Medicine.
Their code of ethics emphasizes principles of morals and ethical conducts.

At ISSCA, their vision is to take a leadership position in promoting excellence and setting standards in the regenerative medicine fields of publication, research, education, training, and certification. ISSCA serves its members through advancements made to the specialty of regenerative medicine. They aim to encourage more physicians to practice regenerative medicine and make it available to benefit patients both nationally and globally.

For more information, please visit us at www.issca.us or send an email to info@stemcellsgroup.com.

Global Stem Cells Group To Start Training Physicians In Lisbon, Portugal as Early as Sep. 2020

The Global Stem Cells Group has at last finalized discussions to open a The Stem Cell Center Network site in Lisbon, portugal. The Stem Cell Center operates an international network of regenerative medicine practitioners that is dedicated to sharing information with the end goal of bringing cutting-edge regenerative medicine-based treatments to patients suffering from debilitating disease. 
 
The new Portuguese Stem Cell Center will be comprised of a highly-accredited, state of the art clinic/laboratory manned by physicians and scientists with combined decades of experience. It will be another strong foothold in Iberia for the global company, and carries with it full license to use the Group’s proprietary treatment methods and branding designs. 
 
This will be the Global Stem Cell Group’s first permanent location in Portugal, rounding out the company’s presence in Western Europe and the Portuguese-speaking market with the new Lisbon-based partnership. The Stem Cell Center Network continues to roll out new membership opportunities, programs, and events, and our membership has grown steadily year after year– To date, we are present in twenty five countries and five continents. 
 
And to commemorate this partnership the Stem Cell Network has plans to open the clinic in September of 2020, and begin training physicians shortly after. “We’ve wanted to expand into Portugal for a very long time,” Said Benito Novas, Founder and CEO, Global Stem Cells Group, “Now that the world is tentatively opening up, it seemed like a good time to resume teaching physicians– after all, with more people getting sick, it is important for their doctors to have all the tools at their fingertips to help,” 
 
Barring any extenuating circumstances, the Stem Cell Center Network remains scheduled to open in the first week of September, with bookings already being accepted for the Network’s inaugural training at this clinic. With limited availability due to current social distancing efforts, spots are filling up quickly, and prospective applicants are encouraged to sign up soon for the hands-on training course. 
 
To Sign Up Today, Visit us At: www.issca.us 
 
About Global Stem Cells Group
Global Stem Cells Group (GSCG) is a worldwide network that combines seven major medical corporations, each focused on furthering scientific and technological advancements to lead cutting-edge stem cell development, treatments, and training. The united efforts of GSCG’s affiliate companies provide medical practitioners with a one-stop hub for stem cell solutions that adhere to the highest medical standards.

Global Stem Cells Group CEO Benito Novas Confirmed Speaker at 1st Ibero-American Fellowship Program in Regenerative and Functional Medicin

The First International Iber-American University Diploma has been announced to focus on laser therapy, hormone therapy, and wellness, and brings with it over a dozen seasoned instructors. The fellowship program, which spans over multiple weeks, covers a variety of topics with the focus of expanding the professional vision of medical professionals, and furthering knowledge of the applications of lasers in gynecology and cosmetic medicine. Additionally, it will update physicians on the newest techniques developed around the world to improve the quality of life of patients. A variety of protocols will be covered, including office, operating room, and hormone-based procedures. 

The Fellowship Program has been certified by Atlantic International University, which is a private university based in Honolulu, Hawaii. Those physicians who complete the fellowship will be asked to demonstrate their knowledge through the use of clinical case studies involving real patients. Additionally, according to Unwritten, AIU offers students the opportunity to supplement this program with resources in various areas of knowledge, at no extra cost. 

Benito Novas, CEO of Global Stem Cells Group, had this to say about the conference: “I am extremely proud and excited to be bringing this conference to physicians around the world. The program has been organized and directed by dear friends of mine, who have put in countless hours to ensure over a dozen physicians from a wide variety of specialties– really, nearly every field of medicine will be represented, and the course has been structured to ensure maximum retention for each and every student of ours,” 

As a result, Global Stem Cells Group and the International Society for Stem Cells Application have both officially endorsed the course as a way for a physician to increase his knowledge in subjects ranging from cosmetic surgery, to marketing strategies, to hormone therapy, and everything in between. To learn more about the First International Ibero-American University Diploma, you may send an email to aiu-curso@amgerweb.com
 
About Global Stem Cells Group
Global Stem Cells Group (GSCG) is a worldwide network that combines seven major medical corporations, each focused on furthering scientific and technological advancements to lead cutting-edge stem cell development, treatments, and training. The united efforts of GSCG’s affiliate companies provide medical practitioners with a one-stop hub for stem cell solutions that adhere to the highest medical standards.